Location: Animal Parasitic Diseases
Project Number: 1245-32000-090-09
Start Date: Dec 12, 2011
End Date: May 30, 2014
Sera will be acquired from a sample of the NHANES III participants aged 6 and over, and tested for Toxoplasma in duplicate by ELISA using an in-house developed test using an 11 kDa sporozoite protein related to embryogenesis (TgERP). Trichinella seroprevalence will be determined using a commercially available test kit which uses T. spiralis excretory/secretory proteins (E/S) as the antigen in an ELISA format. The collected prevalence data for Toxoplasma will be used to determine the predominant route of infection (oocyst versus tissue cysts in meat) from a representative sampling of the U.S. population and ascribe demographic features to those found to be infected. The collected prevalence data for Trichinella will be used to measure the impact of the elimination of Trichinella from the U.S. swine herd on human health and the prevalence of Trichinella infection in the U.S. population.