Project Number: 6402-21310-004-14
Start Date: Jan 01, 2012
End Date: Dec 31, 2012
The DNA segments are to be cloned into a Bacterial Artificial Chromosome (BAC) vector. The BAC libraries are being made by Clemson University Genomics Institute in conjunction with an ongoing National Science Foundation (NSF) project on cotton fiber. DNA sequences will be generated from each end of the DNA fragment and will be recorded as pairs to allow for genome assembly software to use them as paired end reads. The DNA source will be Tm-1 which is a variety of scientific importance to the cotton research community. Clemson University will be producing DNA fingerprints from the BACS and that DNA will be used to generate BAC end DNA sequence of 40,000 BACs. The data will be released following standard ARS policies in regard to DNA sequence information.