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United States Department of Agriculture

Agricultural Research Service

Research Project: Insertional Mutagenesis of Brachypodium Distachyon (Fy 2012-Fy 2014)

Location: Genomics and Gene Discovery

Project Number: 5325-21000-017-17
Project Type: Reimbursable

Start Date: Mar 01, 2012
End Date: Feb 14, 2015

Objective:
The objectives of the project are to 1. Generate 30,000 insertional mutants. 2. Sequence DNA flanking the insertion sites of all mutants to place the insertions in a genomic context. 3. Collaborate with other groups creating T-DNA lines to integrate their lines into our database.

Approach:
Using an Agrobacterium-mediated transformation procedure we will create a collection of insertional mutants in the model grass Brachypodium distachyon. To determine what gene is disrupted in each mutant, we will sequence DNA flanking the insertion site using the known sequence of the inserted DNA as a starting point. This collection will then be used to identify mutations in genes predicted to affect biomass quality and agronomic characteristics of energy crops. The mutant collection will be made publicly available through a BLAST searchable database to allow other researchers to identify knockouts in specific genes.

Last Modified: 7/22/2014
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