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United States Department of Agriculture

Agricultural Research Service

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Research Project: Elucidation of Signaling Pathways That Regulate Ethylene-Induced Leaf and Flower Abscission of Agriculturally Important Plants

Location: Crops Pathology and Genetics Research

Project Number: 2032-21000-022-01
Project Type: Reimbursable

Start Date: Dec 01, 2012
End Date: Nov 30, 2015

Objective:
Utilize Virus-induced gene silencing (VIGS)-based gene functional analysis to characterize the function of identified candidate genes that appear to be involved in regulated leaf and flower abscission.

Approach:
Use shotgun proteomics (LC-MS/MS) to collect data on the entire abscission proteome. Protein quantification will be accomplished using di-methylation and spectral counting. Implement the new knowledge regarding regulation of floral organ abscission in Arabidopsis, in the ethylene-induced abscission of agriculturally important plants, i.e., soybean and tomato. Determine if an IDA signaling path is essential for organ abscission in tomato and soybean and determine its interaction with ethylene and auxin signaling.

Last Modified: 10/25/2014
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