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United States Department of Agriculture

Agricultural Research Service

Research Project: Screening of Potato Germplasm for Genetic Resistance to the Potato Mop-Top Virus

Location: Small Grains and Potato Germplasm Research

Project Number: 2050-21000-032-05
Project Type: Specific Cooperative Agreement

Start Date: Sep 10, 2012
End Date: Aug 31, 2016

Objective:
Screen potato germplasm for Potato Mop-Top Virus (PMTV) resistance in a North Dakota field with a natural infestation of PMTV within the powdery scab fungus (vector of PMTV). Breeding clones identified as resistant will be used for developing genetic resistance to PMTV in potato cultivars. Screen a wide range of potato cultivars in all market classes and Aberdeen potato germplasm for resistance to powdery scab and potato mop-top virus (PMTV) in the field in North Dakota.

Approach:
Five families representing 610 unique breeding clones from the Aberdeen potato breeding program will be screened for resistance to PMTV in a North Dakota field identified as having the necessary infestation for successful evaluation of clonal response to PMTV infection. The five families are derived from European potato cultivars identified as having resistance to PMTV. Clones that demonstrate an adequate level of resistance or tolerance to PMTV will be further investigated in larger, replicated field trials. Susceptibility to tuber necrosis caused by PMTV will be determined by the frequency of tubers expressing symptoms as confirmed by RT-PCR and the severity of internal tuber necrosis observed using a commonly used 0-4 scale. Potato cultivars will be screened for susceptibility to powdery scab and PMTV from all market classes from as wide a genetic base including niche table cultivars recently introduced into the USA from Europe and elsewhere. Germplasm developed from a number of Solanum species, and advanced selections, will initially be evaluated in replicated plots using 10-20 hills per rep. Among the material to be evaluated will be progenies from the use of PMTV resistant cultivars, Teena, Susanna, and Kiva. Families with these three cultivars as parents were produced from hybridizations conducted in Aberdeen, ID in 2009 and 2010. Seedling tubers of four families with Kiva as the parent and one family with Teena as the parent were produced in the greenhouse in 2011 and a preliminary screening for PMTV resistance was conducted in 2012. Seed of progeny of these five families will be increased in the greenhouse and provided for field screening in 2013. Breeding clones that demonstrate an adequate level of resistance or tolerance to PMTV will be further investigated in replicated field trials in following years. Remaining true potato seed of families of Kiva, Teena, and Susanna will be planted and seedling tubers produced in 2013. Susceptibility to tuber necrosis caused by PMTV will be determined by the frequency of tubers expressing symptoms as confirmed by RT-PCR and the severity of internal tuber necrosis observed using a commonly used 0-4 scale.

Last Modified: 12/20/2014
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