Project Number: 6435-43440-044-08
Start Date: Oct 01, 2012
End Date: Jul 31, 2015
Samples of developing Pecan nuts will be extracted to isolate total ribonucleic acid (RNA) and protein. RNA will be sequenced using next-generation sequencing, and then analyzed and annotated to identify specific genes and compare expression profiles. Quantitative-real-time polymerase chain reaction (qRT-PCR) will be done to confirm findings for individual genes. Total protein accumulation during Pecan nut development will be analyzed by gel electrophoresis and mass-spectrometry. Major Pecan allergens Car i 1, Car i 4, and the 7S vicllin will be studied using each of these techniques to characterize their expression, processing, and accumulation. Other Pecan allergens will also be identified based upon homology.