Location: Obesity and Metabolism Research Unit
Project Number: 5306-51530-022-06
Start Date: Dec 01, 2012
End Date: Nov 30, 2014
Fillets of Atlantic salmon fed diets where fish oil was partly (80%) replaced with either rapeseed oil (RO) or soy bean oil (SO) will be used to prepare western diets (WD) for a mouse feeding trial. We will replace 50% of the standard dietary protein source (casein) with proteins from salmon fed FO, RO, or SO (WD-FO, WD-RO and WD-SO, respectively). Due to a large difference in casein and salmon protein digestibility, 50% of the protein in our western-reference diet (WD-Ch) will also be exchanged. A low fat reference group will also be included (LF). Analyses will include: in-depth liver histology; lipidomic profiling of liver and plasma (fatty acids, oxylipins, endocannabioids, and ceramides); pyruvate, glucose, and insulin tolerance tests; fecal bacteria DNA purification, and pyrosequencing (if economically feasible).