IDENTIFICATION AND MODULATION OF FUNCTIONAL PROTEIN ASSOCIATION NETWORKS FOR DROUGHT TOLERANCE IN SWITCHGRASS
Plant, Soil and Nutrition Research
Project Number: 8062-21000-036-14
Start Date: Dec 01, 2012
End Date: Nov 30, 2015
In this project, we propose to investigate the proteomes of leaf and root tissues in response to different levels and duration of drought stress using isobaric stable isotope containing mass tags. There are a number of specific objectives including: 1) To construct protein function networks for stress-regulated proteins; 2) To identify additional transcriptional factors and signal transduction factors that are putative partners of the identified proteins through database-mining strategies; 3) To develop mathematical models for plant growth response to drought stress; 4) To build an integrated model for protein expression and growth properties; 5). To set up a genetic transformation system for switchgrass to produce transgenic plants; 6) To train a team of faculties and students through collaboration with USDA scientist; 7). To develop web-based resources (public data repositories and Twitter, Blog, etc.) to disseminate real-time information to the scientific community and the general public.
Specific activities performed in the ARS labs will include a) a comparative proteomic analysis using an 8-plex iTRAQ strategy of leaf and root tissues from switchgrass subjected to drought stess of different durations.