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United States Department of Agriculture

Agricultural Research Service

Research Project: Management of Arid Land Plant Genetic Resources and Associated Information

Location: San Joaquin Valley Agricultural Sciences Center

Project Number: 2034-21000-011-00
Project Type: Appropriated

Start Date: Mar 14, 2013
End Date: Mar 13, 2018

Objective:
Objective 1:In cooperation with other NPGS sites, efficiently and effectively regenerate and conserve priority plant genetic resources adapted to long-season and/or arid land conditions, and distribute samples and associated information worldwide. Sub-objective 1A - Serve other NPGS sites by regenerating accessions that require long-season/arid growth conditions, and maintain the backup Corylus collection. Sub-objective 1B - Maintain clonal and seed propagated accessions for NALPGRU site-specific crops, emphasizing accessions with low germination, low seed supply, and open-pollinated seed stocks. Develop new regeneration methods as needed. Prepare and send backup seed to the National Center for Genetic Resources Preservation (NCGRP). Objective 2: Strategically expand the genetic diversity in selected germplasm collections and improve associated information for priority long-season and/or arid land plant genetic resources through international and domestic germplasm exchanges and plant explorations. Objective 3: In cooperate with other NPGS sites, characterize and evaluate priority genetic resources for genetic markers, phenotypic descriptors, and key agronomic or horticultural traits. Incorporate characterization and evaluation data into GRIN-Global and/or other databases. Sub-objective 3A - Optimize DNA extraction methodologies and develop molecular markers for Opuntia (prickly pear) and Simmondsia (jojoba) to characterize the collections. Upload marker data to GRIN-Global. Sub-objective 3B - Characterize the Opuntia and Simmondsia collections phenotypically and transfer descriptor data into GRIN-Global.

Approach:
Develop, apply, and follow crop species specific germplasm regeneration protocols supplied by the crop germplasm curators carefully to produce seeds and vegetative propagules as a part of germplasm regeneration and rejuvenation program for different participating NPGS repositories. As a germplasm conservation backup location for Corylus collection from the NCGR, Corvallis, follow all the specified cultivation and management practices for effective management of collection. Strictly follow the crop curators’ protocols to maintain the genetic integrity of each and every accession. Follow appropriate crop rotation and management practices to avoid cross-contamination either through regeneration of residual species from previous years or cross pollination among accessions within and among related outcrossing species. Strictly follow bagging, caging, and apply and maintain appropriate in-cage pollinators during pollination to produce healthy well-filled genetically true-to-type seeds. Maintain the recommended plot size and plant population to avoid harmful effects of genetic drift in outcrossing crop species germplasm and to maintain allelic diversity within and among accessions. All recommended procedures and protocols will be strictly followed to avoid seed contamination while harvesting, processing, and cleaning. Develop proper field maps and label individual accessions while sowing, planting and carefully follow labels while harvesting, processing, and bagging of final seeds. Acquire germplasm, conserve and maintain a broad spectrum of diversity among the in-house germplasm collections assigned. Determine the Minimum Viable Population (MVP) size that is required to avoid erosion of genetic diversity within and among collections through genetic drift in outcrossing seed producing species. Establish methods and protocols to maintain genetic diversity in outcrossing species collections. Characterize germplasm collections using molecular markers and phenotype collections as per standard descriptors. Apply appropriate statistical methods to analyze the genetic diversity, structure, and differentiation to descript genetic diversity and pattern of distribution within and among different taxa. Photodocument morphological diversity within and among different taxa. Upload all data, pictures and results on to Genetic Resources Information Network. Strategically acquire germplasm through plant explorations in the centers of diversity of crop species and through exchange with other collection sites. Plan and execute plant explorations as per the USDA-ARS/NGRL Plant Exchange Office guidelines. Follow strictly all federal and state plant quarantine protocols and Convention of Biological Diversity treaty while plant exploration and importing of germplasm. Distribute appropriate germplasm and associated information to stakeholders nationally and internationally.

Last Modified: 12/19/2014
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