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United States Department of Agriculture

Agricultural Research Service

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Research Project: Development of a Pathogen Detection Early Warning System for Spinach Downy Mildew

Location: Crop Improvement and Protection Research

Project Number: 2038-22000-015-11
Project Type: Trust

Start Date: Jan 01, 2013
End Date: Mar 31, 2014

Objective:
The objectives of the proposed work are to identify target DNA sequences for differentiation of strains of the downy mildew pathogen, Peronospora effusa, and to deploy spore traps to the field for specific detection of the pathogen. The work also entails examination of other potential pathogen inoculum sources, including seed and soil.

Approach:
Isolates of Peronospora effusa will be collected from the field and collaborators. DNA will be extracted and alignments of specific DNA sequences will be performed. Spore traps will be deployed near spinach fields for detection of the pathogen, and DNA extracted from spore trap samples also will be analyzed.

Last Modified: 12/25/2014
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