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United States Department of Agriculture

Agricultural Research Service

Research Project: Increasing Soybean Yield with Exotic Germplasm

Location: Soybean Genomics and Improvement

Project Number: 8042-21000-275-43
Project Type: Reimbursable

Start Date: Jan 01, 2014
End Date: Dec 31, 2014

Objective:
Produce high yielding soybean varieties and experimental lines from wild soybean germplasm and develop new technology to more efficiently select and utilize exotic soybean germplasm for increasing yield via the identification of economically important wild soybean diversity and transfer to applied breeding.

Approach:
Based upon the analysis of the nearly 1,200 wild soybean accessions in the USDA Soybean Germplasm Collection that have been analyzed with more than 50,000 single-nucleotide polymorphism (SNP) DNA markers, a core collection of 110 wild soybeans was identified and a representative core subset of 55 accessions was also identified. Experimental lines will be developed by crossing the wild soybean accessions of the core subset with elite soybean cultivars. At the USDA, Beltsville, whole genome DNA sequence analysis of each of the wild soybean lines in the core subset as well as the elite cultivated soybean parents will be completed. In addition, from each of the cultivated x wild soybean crosses three random F4-derived “control lines” will be developed without any selection for agronomic or seed traits. The whole genome DNA sequence of each of the three control lines from each cross will also be determined at the USDA, Beltsville. Collaborating scientists will select the most agronomically desirable lines from many thousands of progeny from each cross. These highly selected F4-derived lines will be yield tested by the collaborators in local and regional tests to identify lines that are equivalent in yield to the best cultivars available. At the USDA, Beltsville, whole genome DNA sequence data will be collected from the three highest yielding lines from each cultivated X wild soybean cross. The whole genome DNA sequence data of the wild soybean and elite cultivated soybean parents as well as the whole genome DNA sequence data from the three control lines and the three highest yielding lines from each cross will be aligned and compared to identify regions of the genome and genes that must be present in order to produce high yielding lines. In addition, unique regions in the high yielding lines derived from the wild soybean parent that lead to yield increases will be identified. The genome positions of these unique yield quantitative trait loci (QTL) will be precisely mapped and SNP DNA markers will be made available to select for these yield QTL.

Last Modified: 10/22/2014
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