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United States Department of Agriculture

Agricultural Research Service

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Research Project: Prevalence and Characterization of Escherichia Coli 0157:h7 Recovered from Retail Ground Neal Within Mid-Atlantic Region of the U.S. -Cfda 10.310

Location: Food Safety and Intervention Technologies

Project Number: 8072-41420-015-15
Project Type: General Cooperative Agreement

Start Date: Feb 20, 2014
End Date: Dec 31, 2015

Objective:
The overall goal of the project is to determine the prevalence, pathogenic potential, and characterization of E. coli O157:H7 in retail ground veal in four states of the Mid-Atlantic region of the United States. The specific objectives are to: 1) determine the prevalence of E. coli O157:H7 in retail ground veal collected from Maryland, Delaware, Pennsylvania, and Virginia; 2) determine the presence of virulence genes (stx1, stx2, eae, ehxA, and others to be determined) in E. coli O157:H7 isolates; 3) characterize E. coli O157:H7 isolates by genotypic (pulsed-field gel electrophoresis) methods; and 4) share meat samples, enrichment broths, and isolates with STEC CAP collaborators for further testing for USDA-FSIS “big six” adulterant serogroups of non-O157 STEC.

Approach:
We are planning to analyze a total of 1,000 raw ground veal samples (250 from each state, at least 1 kg per sample) collected from retail stores in Maryland, Delaware, Pennsylvania, and Virginia over a period of nine months for detection of E. coli O157:H7 and non-O157 in veal. In addition, isolates recovered from positive samples will be tested for virulence genes and PFGE profiles.

Last Modified: 12/17/2014
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