Location: Sunflower Research
Project Number: 5442-21220-028-23
Start Date: Jun 01, 2013
End Date: May 31, 2014
We have a diverse natural population of 160 isolates of S. sclerotiorum collected from various crops in 24 states from the west to the east coast of the United States. These diverse isolates will be genotyped using the Ion Torrent Personal Genomics Machine (PGM). We have modified and adapted a restriction site associated DNA mapping method, a form of genotype by sequencing and optimized it to run on the PMG. We expect to identify 10,000 single nucleotide polymorphisms (SNP) at unique loci across the 38 Mb genome of S. sclerotiorum. This would place a molecular marker about every 4 kb in each of the 160 isolates. This density of SNP markers would facilitate high-resolution association mapping to identify genes underlying loci contributing to phenotypic variation. Generation of a genetic database with free access to this genotyping data will greatly benefit the research community and provide a tool that is essential to utilize the publicly available S. sclerotiorum genome sequence.