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United States Department of Agriculture

Agricultural Research Service

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Research Project: Detection and Identification of Soil-Borne Plant Pathogens and Mitigation of Soil-Borne Diseases

Location: Floral and Nursery Plants Research Unit

Project Number: 1230-22000-049-00
Project Type: Appropriated

Start Date: Apr 29, 2014
End Date: Aug 31, 2017

Objective:
Objective 1: Develop molecular tools to detect, identify, characterize, and mitigate the deleterious effects of soil-borne pathogens, including Rhizoctonia solani, in ornamentals, vegetables, and turf grasses. (NP303, C4, PS 4C). Sub-objective 1A: Identify and differentiate Rhizoctonia (s. lato) pathogens by developing genome fingerprint-based markers and molecular hybridization assays. Sub-objective 1B: Identify and decipher the biology and pathology of Rhizoctonia solani using "omics"-based approaches. 1B.1 Analyze the genomes, transcriptomes and proteomes of soil-borne fungi, including Rhizoctonia solani to catalog genes, which will aide in pathogen identification and characterization of genes with roles in fungal biology, ecology, virulence, and resistance, and 1B.2 Discover and develop novel molecular markers for future investigations on host specificity, fungicide resistance, phylogenic characterization and identification of Rhizoctonia solani. Sub-objective 1C: Determine the sensitivity of molecularly identified pathogenic isolates of Rhizoctonia (s. lato) to commonly used fungicides to formulate effective disease management.

Approach:
Apply appropriate molecular markers identified to molecularly identify anastomosis groups (AGs) of Rhizoctonia solani and develop in vitro assays to determine fungicide sensitivity of different AGs affecting turfgrasses, in order to allow selection of appropriate materials for effective disease control.Identify various isolates of Rhizoctonia solani pathogenic to ornamentals, vegetables, and turfgrasses to the levels of anastomosis groups (AGs) and subgroups by developing UP-PCR genome fingerprint-based, sequence-characterized amplified region (SCAR) markers and cross-blot hybridization assays. Using high throughput molecular methods, analyze the genomes, transcriptomes and 2-dimensional proteomes of selected pathogenic AGs of R. solani to catalog genes and develop microsatellite markers, which will aide in pathogen identification and characterization of genes with roles in host specificity, fungal biology, overwintering, virulence, and fungicide resistance. Develop in vitro assays following the "poisoned agar" technique to determine sensitivity of molecularly identified AGs and subgroups of R. solani to commonly used fungicides, in order to allow selection of appropriate fungicides for effective disease control.

Last Modified: 9/2/2014
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