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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Genetics and Animal Breeding » Research » Publications at this Location » Publication #315049
Title: A novel antimicrobial agent differentially impacts the immunological response of stimulated bovine monocytes

Author
item DAWES, MAISIE - Western University Of Health Sciences
item AGUILAR, JOSE - Western University Of Health Sciences
item Chitko-Mckown, Carol

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/2/2015
Publication Date: 5/11/2015
Citation: Dawes, M.E., Aguilar, J.S., Chitko-Mckown, C.G. 2015. A novel antimicrobial agent differentially impacts the immunological response of stimulated bovine monocytes [abstract]. Immunology 2015, The American Association of Immunologists Annual Meeting, May 8-12, 2015, New Orleans, LA. Poster #193.12.

Interpretive Summary:

Technical Abstract: Despite advances in anti-inflammatory therapy, the US cattle industry now loses over $1 billion annually due to Gram-negative infections. In light of reduced lymphocyte proliferation concurrent with decreased cyclooxygenase-2 and matrix metalloproteinase-9 expression in bovine lactoferrin (bLF)-supplemented cells, we intended to show that bLF and the lactoferricin B (LFcin B) peptide, proven antibiotic agents, can also serve as low-risk anti-inflammatory agents, and hypothesized their ability to influence p38 MAPK signaling which is critical in macrophage-mediated inflammation. While LF is present in neutrophils, it is anticipated that enzymatic activity within infective sites will heighten local LFcin B concentrations. Following pre-treatment with either bLF, the selective p38 MAPK inhibitor SB203580 (SB), or LFcin B, monocytes isolated from healthy 1 wk to 5 mo old cattle stimulated with either lipopolysaccharide (LPS) or anisomycin (Aniso), were evaluated for iNOS, IL-1ß and TNF-a expression. Preliminary data show that like SB, both bLF and LFcin B significantly abrogate LPS- and Aniso-induced TNF- a and IL-1ß expression. In contrast, iNOS expression was increased in cells co-cultured with either LPS or Aniso and each of the three inhibitors. While these results support our hypothesis, additional work is needed to decipher the mechanistic rationale for the immunomodulatory effects of bLF and LFcin B, and the basis for differential cytokine release.