Author
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NADELLA, DURGA - NORTH DAKOTA STATE UNIV |
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Hoffer, Barry |
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Foley, Michael |
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Submitted to: Weed Science Society of America Meeting Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 12/13/2002 Publication Date: 2/8/2002 Citation: Nadella, D., Hoffer, B.L., Foley, M.E. 2003. Identification of quantitative trait loci (QTLs) for dormancy in wild oat (Avena fatua). Weed Science Society of America Meeting. February 8-14, 2003. Jacksonville, FL. 43:27. Interpretive Summary: Technical Abstract: Wild oat is a wide spread weed causing substantial yield losses in small grain crops. Variable germination of seeds due to dormancy makes the control of wild oat difficult. Dormancy is a complex polygenic character with large influences of the environment on its expression. We are investigating genetic and environmental factors regulating dormancy in wild oat. To this end, a genetic linkage map is being constructed on 126 F2:F9 recombinant inbred (RI) lines derived from the cross M73 (D) x SH430 (ND) to detect dormancy quantitative trait loci (QTLs). So far, 255 amplified fragment length polymorphism (AFLP) loci have been mapped and 28 linkage groups have been identified covering a map length of 1560 cM. Including the 16 unlinked loci, the genome coverage is comparable to that of the cultivated hexaploid oat (Avena sativa). The linkage groups varied in their length from 1.6 cM with 2 loci to 220 cM with 52 loci. A significant effect of temperature on germinability and a genotype by germination temperature interaction have been detected over a range of 10 to 30 C in the RI population. Using bulked segregant analysis, 45 loci showing differential fragment intensities between the dormant and non-dormant bulks have been identified at 15 C. Except for a few, most of the QTLs are sensitive to the germination temperature and are differentially expressed. The interaction of all of these dormancy QTLs with germination temperature is being investigated. foleym@fargo.ars.usda.gov |
