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ARS Home » Research » Publications at this Location » Publication #215950
Title: Utility of proteomics techniques for assessing protein expression

Author
item Natarajan, Savithiry - Savi
item XU, CHENPING - UNIVERSITY OF MARYLAND
item Cregan, Perry
item Caperna, Thomas
item Garrett, Wesley
item Luthria, Devanand - Dave

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 10/23/2007
Publication Date: 10/25/2007
Citation: Natarajan, S.S., Xu, C., Cregan, P.B., Caperna, T.J., Garrett, W.M., Luthria, D.L. 2007. Utility of proteomics techniques for assessing protein expression. Meeting Abstract. p. 51-55.

Interpretive Summary:

Technical Abstract: In recent years, proteomic technologies have been frequently used as an effective analytical tool for examining modifications of protein profiles for accessing the bio-safety of genetically modified crops (GMO). Understanding of natural variation of soybean seed proteins is critical for determining unintended collateral effects in GMO soybeans. We used two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), matrix-assisted laser desorption/ionization time of flight (MALDI-TOF), and liquid chromatography mass spectrometry (LC-MS) for the separation, quantification, and identification of different classes of soybean seed proteins. In this study, sixteen soybean genotypes including four wild and twelve cultivated belonging to four different subgroups were used as models for protein profile evaluation. Significant variations of allergen and anti-nutritional protein profiles were observed in cultivated and wild soybean genotypes. However, a minor variation of protein profiles was observed within the same subgroup.