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Title: VitisGen on the road: mapping the way to the next generation of grapes

Author
item YANG, SHANSHAN - Cornell University
item FRESNEDO, JONATHAN - Cornell University
item TAKACS, ELIZABETH - Cornell University
item BARBA, PAOLA - Cornell University
item HYMA, KATIE - Cornell University
item Lillis, Jacquelyn
item ACHARYA, CHARLOTTE - Cornell University
item FISHER, ANN - Cornell University
item COTE, LINDA - Cornell University
item MANNS, DAVID - Cornell University
item RYONA, IMELDA - Cornell University
item GADOURY, DAVID - Cornell University
item SEEM, BOB - Cornell University
item SACKS, GAVIN - Cornell University
item MANSFIELD, ANNA KATHERINE - Cornell University
item Ledbetter, Craig
item LUBY, JIM - University Of Minnesota
item HEMSTAD, PETER - University Of Minnesota
item FENNELL, ANNE - South Dakota State University
item HWANG, CHIN-FENG - Missouri State University
item WALKER, ANDY - University Of California
item RIAZ, SUMMAIRA - University Of California
item COUSINS, PETER - E & J Gallo Winery
item Londo, Jason
item Buckler, Edward - Ed
item MITCHELL, SHARON - Cornell University
item SCHWEITZER, PETER - Cornell University
item SUN, QI - Cornell University
item Cadle-Davidson, Lance
item REISCH, BRUCE - Cornell University

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/16/2014
Publication Date: 1/1/2015
Publication URL: https://pag.confex.com/pag/xxiii/webprogram/Paper15597.html
Citation: Yang, S., Fresnedo, J., Takacs, E., Barba, P., Hyma, K., Lillis, J.A., Acharya, C., Fisher, A., Cote, L., Manns, D., Ryona, I., Gadoury, D., Seem, B., Sacks, G., Mansfield, A., Ledbetter, C.A., Luby, J., Hemstad, P., Fennell, A., Hwang, C., Walker, A., Riaz, S., Cousins, P., Londo, J.P., Buckler Iv, E.S., Mitchell, S., Schweitzer, P., Sun, Q., Cadle Davidson, L.E., Reisch, B. 2015. VitisGen on the road: mapping the way to the next generation of grapes. Annual International Plant & Animal Genome Conference. P0873: 15597.

Interpretive Summary:

Technical Abstract: The USDA-NIFA VitisGen project (Award No. 2011-51181-30635) aims to optimize grape cultivar improvement through the integration of grape breeding, cutting edge genomics technology, precision phenotyping, socioeconomic research, and extension. VitisGen is a five-year project involving 25 co-PIs at 11 institutions, focused on identifying the genes associated with economically important traits and on developing tools for marker-assisted selection (MAS). To date, 23,328 vines were genotyped for marker development or application. Seventeen F1 families were prioritized as core sets to develop new markers. Genotyping-by-sequencing (GBS) technology was applied to obtain single nucleotide polymorphism (SNP) markers, which were used for linkage map construction and subsequent quantitative trait locus (QTL) mapping. Three trait evaluation centers provided quantitative phenotypic data for analysis of powdery mildew resistance, low temperature response, and fruit quality. The average number of raw SNP markers per population was 150,000, of which 10% with high quality were chosen on average for linkage mapping. Twenty-two parental maps were generated from eleven crosses and utilized for QTL mapping. At least one major QTL was identified for each trait. Further analyses such as candidate gene scans and PCR marker development are in process. In the meantime, the project developed a high-throughput protocol for SSR processing and analysis, which allowed breeders to utilize MAS to increase the efficiency of seedling trials. The results so far support the VitisGen vision to pursue a better understanding of grape genomics and to implement molecular procedures to accelerate the breeding process, which will ultimately benefit researchers, breeders, growers, and consumers.