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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #80073
Title: 1,25-DIHYDROXYVITAMIN D3 INHIBITS INTERFERON-GAMMA (IFN-GAMMA) SECRETION LECTIN-STIMULATED MONONUCLEAR LEUKOCYTES FROM DAIRY CATTLE

Author
item AMETAJ, BURIM - IOWA STATE UNIVERSITY
item Nonnecke, Brian
item Reinhardt, Timothy
item BEITZ, DONALD - IOWA STATE UNIVERSITY

Submitted to: International Virtual Conference on Infectious Diseases of Animals
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We have shown that 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], the most potent biological metabolite of vitamin D, modulates the proliferation and differentiation of bovine CD4+ and CD8+ PMNL in vitro. To further our understanding of this compound's effects on bovine PMNL function, the effect of 1,25-(OH)2D3 and two synthetic analogues, 22-26-F3-1,25-(OH)2D3 and 1,25,28-(OH)3D2, on IFN-gamma secretion by PMNL from ovalbumin (OVA)-sensitized adult dairy cattle were evaluated. Mononuclear leukocyte cultures were unstimulated or stimulated with pokeweed mitogen (5 and 10 mg/ml); OVA (16 and 32 mg/ml) or lipopolysaccharide (LPS, 10 mg/ml). Vitamin D concentrations in cultures were 0, 10**-10, 10**-9, 10**-8 moles/L. Culture supernatants were harvested at 24 and 48h and analyzed for IFN-gamma using an IFN-gamma-capture ELISA. Interferon-gamma secretion by resting and LPS-stimulated cultures was undetectable in control and vitamin D-supplemented cultures. 1,25-(OH)2D3, at 10**-9 and 10**-8 moles/L, inhibited (P<.05) secretion by PWM-stimulated cells; however, the synthetic analogue, 22-26-F3-1,25-(OH)2D3 at 10**-10 to 10**-8 moles/L inhibited (P<.001) IFN-gamma secretion by PWM-stimulated cells under all culture conditions.