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Title: REDUCED VIRULENCE OF A BORDETELLA BRONCHISEPTICA SIDEROPHORE MUTANT IN NEONATAL SWINE (POSTER PRESENTATION FOR THE 99TH ASM MEETING, MAY 30- JUNE 3, 1999)

Author
item Register, Karen
item DUCEY, T - UNIV. OK, OKLAHOMA CITY
item Brockmeier, Susan
item DYER, DAVID - UNIV. OK, OKLAHOMA CITY

Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: 2/22/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: B. bronchiseptica acquires iron through production of the siderophore alcaligen. A nonrevertable alcaligin mutant of the virulent swine strain 4609 (DBB25) was constructed by insertion of a kanamycin resistance cassette into alcA, one of the genes essential for alcaligen biosynthesis. In two experiments, the virulence of the alcA mutant in colostrum-deprived, ,Caesarean-derived piglets was compared with that of the parent strain. At one week of age piglets were inoculated with PBS, 4609, or DBB25 (1 x 10**6 CFU; N=6/group in each experiment). Two piglets in each group were euthanized on day 10 post-infection (PI); remaining piglets were euthanized 21 days PI. Respiratory tissues were harvested for quantitative culture and histologic examination. Nasal washes performed 7, 14, and 21 days PI demonstrated significantly reduced colonization of the nasal cavity by DBB25 as compared with 4609 (p