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United States Department of Agriculture

Agricultural Research Service

Title: Rapid Isocratic Hplc Analysis of Hypericins

item Gibson, Donna

Submitted to: International Botanical Congress
Publication Type: Abstract Only
Publication Acceptance Date: July 2, 1999
Publication Date: N/A

Technical Abstract: We describe a new method for the analysis of hypericins from crude extracts of Hypericum perforatum (St. John's wort) using HPLC. Hypericin and pseudohypericin and related compounds were separated in 15 minutes using a simple isocratic elution method on a modified phenyl column. This isocratic method permits the quantitative and rapid analysis of hypericins in crude acetone samples. In addition, we report on the photoconversion of protohypericins to hypericins, as well as the stability of hypericin and pseudohypericin as a function of light, time, and storage conditions.

Last Modified: 4/21/2015
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