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United States Department of Agriculture

Agricultural Research Service

Title: Genetic Variation for 15 Isozyme Loci Between Chinese and North American Soybean Accessions

item Nelson, Randall
item Palmer, Reid

Submitted to: Soybean Research World Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: April 15, 1999
Publication Date: N/A

Technical Abstract: One hundred and eight soybean accessions representing the major progenitors and their derived cultivars from China and US were examined for a total of 17 loci including physiological trait (root fluorescence, Fr), morphological trait (pubescence tip, Pb), and 12 isozyme systems. Isozyme assays were conducted for aconitase (Aco), acid phosphatase(Ap), diaphorase (Dia), endopeptidase (Enp), seedcoat peroxidase (Ep), isocitrat dehydrogenase (Idh), leucine aminopeptidase (Lap), malate dehydrogenase (MDH), malic enzyme (ME), phosphogluconate dehydrogenase (Pgd), phosphoglucose isomerase (Pgi), and phosphoglucomutase (Pgm). A total of 40 alleles was observed for 17 loci among US and Chinese accessions. Except two monomorphic loci of Fr and Pb, each of Aco2, Enp, Idh1, Idh2, Lap, MDH, Pgi and Pgm loci had two alleles, and the other loci had three alleles. The alleles of Aco4-a and Ap-c were not present in Chinese accessions, while Aco3-b, Aco3-c, Enp-c and ME-c alleles were absent in th US accessions. However, the allelic frequencies of the accessions between two countries were similar for the Aco, Ap, Enp, Ep, Fr, MDH, ME and Pgd loci. For the Dia1, Idh1, Idh2, Lap, Pgi and Pgm loci the same alleles were present but the allelic frequencies were different and the predominant allele at each locus was different between the US and Chinese accessions.

Last Modified: 4/21/2015
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