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United States Department of Agriculture

Agricultural Research Service

Title: Combined Aflp and Rflp Mapping in Two Hexaploid Oat Recombinant Inbred Populations

Authors
item Jin, Hua - UNIV OF ILLINOIS
item Domier, Leslie
item Shen, X - UNIV OF ALABAMA
item Kolb, Frederic - UNIV OF ILLINOIS

Submitted to: Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 17, 1999
Publication Date: N/A

Interpretive Summary: The identification of molecular markers linked to agronomically important genes is an important tool in understanding the physiology of a particular trait and the production of more productive plant lines with enhanced disease resistance. As more and more molecular markers are identified, it is possible to construct maps of plant chromosomes, which makes the identification of new genes and chromosomal regions easier and more precise. However, the chromosomes of a single pair of parental plants do not contain all genes for all traits. Consequently it is often necessary to cross different pairs of plants to study particular traits. Unfortunately, this usually means that a new set of marker information must be generated. In these studies, we examined the relationship between molecular markers that we identified in groups of plants produced by crossing two different set of parental oat lines. One of the groups of oat lines had many markers assigned and the other had none. We used a technique that allowed us to rapidly identify new markers in both populations and showed that we could relate the chromosomes of the two groups of oat plants and the markers previously identified in the first group of plants. The information will aide other scientist in their efforts to identify chromosomal regions that contain genes and more quickly relate their finding to previously mapped genes in oat and other grass species, which in turn will, lead the more rapid development of new and improved oat lines.

Technical Abstract: A combined RFLP and AFLP map was constructed for hexaploid oat. The segregation of AFLP markers was scored in two hexaploid oat recombinant inbred line populations, the Kanota x Ogle RFLP population and a population derived from Clintland64 and IL86-5698, barley yellow dwarf virus sensitive and tolerant lines, respectively. More than 300 AFLP markers were scored in each population, of which 97 could be scored in both populations. AFLP markers were linked to RFLP markers in 32 of 36 Kanota x Ogle RFLP linkage groups. The addition of the AFLP markers to the Kanota x Ogle RFLP data set combined markers from four pairs of linkage groups and expanded the map from 2530 cM to 3707 cM. Thirty linkage groups were observed in the Clintland64 x IL86-5698 population, two of which could be consolidated by comparing the maps from both populations. The AFLP and RFLP markers showed very similar distributions in the Kanota x Ogle population. The placement of a set of AFLP markers on the Kanota x Ogle linkage map will enrich the RFLP map and allow others to relate AFLP markers for agronomically important genes to the reference Kanota x Ogle linkage map.

Last Modified: 10/21/2014
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