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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of Two Types of Plating Media for Detection and Enumeration of Campylobacter from Poultry Samples

Authors
item Berrang, Mark
item Line, John

Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: August 10, 1999
Publication Date: September 12, 1999
Citation: BERRANG, M.E., LINE, J.E. COMPARISON OF TWO TYPES OF PLATING MEDIA FOR DETECTION AND ENUMERATION OF CAMPYLOBACTER FROM POULTRY SAMPLES. CAMPYLOBACTER HELICOBACTER AND RELATED ORGANISMS INTERNATIONAL WORKSHOP. 1999.

Technical Abstract: A newly developed media, Campy-Line agar, was compared to Campy- Cefex agar for recovery of Campylobacter spp. Broiler carcasses were collected from a commercial processing plant. Five samples were examined from each carcass including: feathers, skin, crop, ceca and colon. All samples including surface contamination or internal contents were placed into sealable plastic bags, diluted and serial dilutions were plated onto each media. Campy-Line agar provided Campylobacter spp. counts that closely mirrored those found by enumeration on Campy-Cefex agar. Pearson correlations were conducted. Campylobacter populations recovered from feather and skin samples using Campy-Line media were correlated with those recovered using Campy-Cefex agar with r values of .94 and .95 respectively. The best correlations were found with crop samples with an r of .98 and an r2 value of .95, indicating that 95% of the variation seen in the Campy-Line counts was seen in counts from Campy-Cefex agar. The lowest correlation was found with the ceca and colon with r values of .87 and .88 respectively. Subjective observations suggest that Campy-Line media is easier to use due to the virtual absence of contaminating colonies. These colonies can mask Campylobacter, making enumeration a challenging and tedious task. With the high level of correlation measured between counts from the two types of media, it is recommended that the ease of use of Campy-Line media be taken into account when choosing a selective plating media for Campylobacter from poultry samples.

Last Modified: 11/23/2014
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