Submitted to: Recent Research Developments in Agricultural and Food Chemistry
Publication Type: Book / Chapter
Publication Acceptance Date: September 13, 1999
Publication Date: August 28, 2000
Technical Abstract: This is a review of the immunoassays used for detection of various antibiotics in foods and feed. Screening methods are needed for monitoring anti-microbial residues in foods and feeds because of their widespread use in modern agriculture. Immunoassays are rapid, highly sensitive analysis tools that fulfill the need for rapid analysis and screening of antibiotic residues. Immunoassay formats and sources of method variability are discussed. Regulatory aspects, quality and control, as well as statistical applications used with immunoassays are also presented. A table listing antibody production and immunoassay development for over sixty antibiotics in seventeen different chemical classes is provided. Computer modeling studies which have been used to guide and shape the understanding of antibody antigen binding and hapten antigen similarities are discussed. A review is presented of the immunoassay studies conducted in our laboratory on the antibiotics ceftiofur, dimetridazole, halofuginone, hygromycin B, monensin, narisin, nicarbazin, salinomycin, sarafloxacin, and sulfadimethoxine. New technologies that use antibodies as the heart of the method also are being investigated in our laboratory. These technologies include electrochemiluminescence (ECL) detection using the ORIGEN Analyzer, and immunoaffinity capture coupled to LC. The production of molecularly imprinted polymers (MIPs) or plastic anti-bodies may also be used to bind chemicals. MIPs were used in a molecularly imprinted sorbent assay (MIA) and a molecularly imprinted solid phase extraction (MISPE). This compilation of work on the detection of antibiotics by immunoassay and various antibody applications demonstrate the broad range of application of the ELISA methodology.