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United States Department of Agriculture

Agricultural Research Service

Title: A Rapid Method for the Determination of Trace Levels of Nonylphenol in Fishtissue Using Solid Phase Extraction and Hplc Fluorescence Detection

Authors
item Datta, Seema - FAS
item Loyo, J - USDA/ARS
item Rice, Clifford

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 25, 1999
Publication Date: N/A

Technical Abstract: A simple automated extraction method for the determination of alkylphenols in fish tissue is reported. An automated apparatus is used to solvent extract ground fish tissue and the resulting extract is cleaned-up on an aminopropyl silica (APS) extraction cartridge. With no further sample preparation, nonylphenol is quantitated using a normal phase HPLC, APS Hypersil column, with fluorescence detection. The major advantage of this technique is the elimination of the conventional gel-permeation step, a lengthy clean-up procedure designed to remove lipids. Representative fish from clean sites and contaminated sites were analyzed for nonylphenol. Lake trout hatchery reared fish and yellow perch from Milwaukee Bay ranged from 30 to 200 nonylphenol ng/g wet weight.

Last Modified: 12/20/2014
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