Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: May 25, 2000
Publication Date: N/A
Technical Abstract: Phylogenetic analysis of the genera within the suborder Pseudonocardineae based on almost complete sequences of 16S rDNA showed that Saccharothrix cryophilis NRRL B-16238 was misplaced within the genus Saccharothrix. This species, along with the genera 'Actinoalloteichus', Kutzneria, and Streptoalloteichus, appears to be phylogenetically outside of both the family Actinosynnemataceae and family Pseudonocardiaceae. S. cryophilis NRRL B-16238 appears to be phylogenetically closest to Streptoalloteichus, but is morphologically distinct from this genus because sporangia with motile spores are not observed. The aerial mycelium fragments into rod- shaped elements and sclerotium-like bodies are occasionally observed in the substrate mycelium. The cell wall contains meso-diaminopimelic acid, whole cell hydrolysates contain galactose, rhamnose and ribose, phospholipid pattern is type PIV, and the principal menaquinone is MK-9(H4). A new genus is proposed for this species based on its chemotaxonomy, morphology, and phylogenetic position and is to be called Crossiella in recognition of the contributions of Thomas Cross, a distinguished actinomycete biologist at the University of Bradford. The type species is Crossiella cryophilis gen. nov., comb. nov.