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United States Department of Agriculture

Agricultural Research Service

Title: Induction of Highly Embryogenic Calli and Plant Regeneration in Diploid and Tetraploid Cottons

item Sakhanokho, H - ALABAMA A&M UNIVERSITY
item Karaca, Mehmet - MISSISSIPPI STATE UNIV
item Rajasekaran, Kanniah
item Saha, Sukumar

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: January 4, 2000
Publication Date: N/A

Technical Abstract: There is a need to broaden the number of regenerable cotton lines. Through a combination of technique, media and strict timing, a methodology has been developed to produce consid- erable amounts of somatic embryos(SEs)in two tetraploid and two diploid accessions. Callus was initiated from hypocotyl or cotyledon explants and transferred within a critical time frame to a callus proliferation/maintenance medium. Potentially embryogenic calli were then transferred to liquid culture for four weeks, strained through a mesh screen and placed on an embryo development/maturation medium. High numbers of somatic embryos were reproducibly cultivated from all these lines. Mature SEs placed on medium with no hormones germinated and produced a few plants. Efforts were undertaken to improve the plant regeneration efficiency by a combination of media composi- tion and time of exposure. In addition, genetic changes due to the culturing process, somaclonal variation, were also investigated in a preliminary study. Preliminary micro- molecular analysis, using fluorescently-labeled AFLP primer pairs, indicated little somaclonal variation due to the culturing process. This report offers,for the first time, a series of regenerable and highly embryogenic lines that can be developed into future generations of transgenic cottons. Also, methodology followed has the potential for improving embryogenicity of other recalcitrant cotton lines.

Last Modified: 4/19/2015
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