|Hodkova, M - CZECH ACADEMY OF SCIENCES|
|Okuda, T - JAP MIN AGRI FORES & FISH|
Submitted to: In Vitro Cellular And Developmental Biology
Publication Type: Abstract Only
Publication Acceptance Date: March 1, 2000
Publication Date: N/A
Technical Abstract: Activity of corpora allata (CA) of the fire bug, Pyrrhocoris apterus (Heteroptera), was determined in vitro by measurement of the radioactive nonpolar compounds released into incubation medium containing 3H-methionine. The CA of adult females had little or no activity. Supplementation of farnesol specifically enhanced radioactivity in an unknown product (Un) that is different from the known juvenile hormones (JHs). The rate of production of Un by the CA in vitro was correlated with the effect of the CA on ovarian maturation in vivo. Therefore the Un could be a good candidate for the heteropteran JH that has not yet been identified. In vitro production of Un is regulated by the pars intercerebralis of the brain. Based on surgical interventions to the neuroendocrine system in vivo and in vitro, slow (irreversible in vitro) and fast (reversible in vitro) regulation of the CA can be distinguished. The slow stimulation or inhibition is associated with regulation of the CA volume. The fast inhibition is produced by the intact brain, while the fast stimulation is only produced by the brain extract.