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United States Department of Agriculture

Agricultural Research Service

Title: Removal of Salmonella Enteritidis from Room Environments Using Negative Air Ionization - Possible Role of Bactericidal Activities by the Ionizer in This Removal

item Seo, Kun Ho
item Holt, Peter
item Mitchell, Bailey
item Gast, Richard

Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: February 22, 2000
Publication Date: May 15, 2000
Citation: Seo, K., Holt, P.S., Mitchell, B.W., Gast, R.K. 2000. Removal of salmonella enteritidis from room environments using negative air ionization - possible role of bactericidal activities by the ionizer in this removal. American Society for Microbiology.

Technical Abstract: Studies have demonstrated that transmission of Salmonella Enteritidis (SE) within a flock could occur via the airborne route, especially in stressed birds. To investigate whether the ionizer exerted a bactericidal effect on the organisms, a sealed glass chamber was constructed into which an aerosol containing SE was pumped. On the walls, ceiling, and floor of the chamber were attached XLT4 plates which were exposed to the aerosol for 3 hours, i the presence or absence of the ionizer. The plates were removed from the chamber, incubated at 37C for 24 hours, and then counts were made. Greater than 1000 CFU/plate were observed on plates exposed to the aerosol without the ionizer compared with less than 20 CFU/plate on the ionizer-exposed plates. Experiments where, after being exposed to the 3 hour aerosol, the inside surfaces of the chamber were rinsed with 100 ml PBS and the rinse plated onto XLT4 plates, showed that, while the rinse from the chamber exposed to the aerosol in the absence of the ionizer contained colony counts greater than 150 CFU/ml of wash, no colonies were found in the rinse from ionizer-exposed chambers. These results indicate that negative air ionization can have a significant impact on the airborne microbial load in a poultry house and at least a portion of this effect is through direct killing of the organisms.

Last Modified: 4/19/2015
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