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United States Department of Agriculture

Agricultural Research Service

Title: Evaluation of Immuno-Concentration Procedure to Detect Salmonellae in Poultry Samples

Authors
item Bailey, Joseph
item Cosby, Douglas

Submitted to: International Association of Milk Food and Environmental Sanitarians
Publication Type: Abstract Only
Publication Acceptance Date: January 25, 2000
Publication Date: N/A

Technical Abstract: Two immuno-concentration methods were compared to the USDA, FSIS cultural method for the detection of salmonellae in processed chicken rinse and poultry feed samples. The VIDAS Immuno- Concentration Salmonella (ICS) assay is an automated process for the selective enrichment of salmonellae for use on the VIDAS system. Both ICS procedures and the USDA procedure begin with the sample being preenriched in buffered peptone water for 18 to 24 hr. Following preenrichment and ICA immuno-concentration, test samples were analyzed by two separate methods. For Method 1, released salmonellae were post enriched in a non-selective growth medium for 5-6 hr, followed by immunoassay detection (24- 30 hr test). For Method 2, released salmonellae were plated on designated selective agar plates (42 to 48 hr test). The USDA, FSIS method is a 72 hour procedure. In these studies, 3 replications of 20 broiler carcass rinse samples and 1 set of 20 inoculated feed samples were evaluated by each of the methods. For broiler rinse samples, 12/60 (20%) and 9/60 (15%) of sampes were found positive by methods 1 and 2, respectively compared to 11/60 (18%) by the USDA, FSIS procedure. One hundred percent (20/20) of inoculated feed samples were positive by all methods. The VIDAS ICS followed by automated ELISA is an automated procedure which accurately detected salmonellae from poultry samples in 24 hours, saving 1 to 2 days over other traditional ELISA and cultural procedures.

Last Modified: 12/25/2014
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