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Title: MOLECULAR AND MORPHOLOGICAL CHARACTERIZATION OF THREE NEW PRATYLENCHUS SPECIES

Author
item Carta, Lynn
item Skantar, Andrea
item Handoo, Zafar

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: 6/23/2000
Publication Date: 12/1/2000
Citation: Carta, L.K., Skantar, A.M., Handoo, Z.A. 2000. Molecular and morphological characterization of three new pratylenchus species. Journal of Nematology 32: 423.

Interpretive Summary:

Technical Abstract: Morphometrics and photographs of three new species and their proposed relationships to other Pratylenchus species using 28SrDNA are presented. Two new South African species from cotton are related to one another morphologically, differing in length of stylet and postvulval sac, presence or absence of lateral line crenations and tail projections, number of tail annules, shape of stylet knobs and median bulb, and behavior. Their 28SrDNA sequences differ by only 2 deletions/300 bp (base pairs) from one another, and by 16/300 bp from Nacobbus aberrans. The closest morphological relative is P. teres with 6 lateral lines. A new species from grass in Arlington Cemetery, Virginia, U.S.A., has the unusual morphological character among Pratylenchus species of 6-8 crenate lateral lines and a pyriform to slightly overlapping basal bulb. Molecularly, the 28SrDNA sequence of the Arlington Cemetery species is closely related to P. convallariae (3/300 bp) and distantly to P. hexincisus (45/300 bp).