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Title: GENETIC MANIPULATION OF A VERY VIRULENT STRAIN OF MAREK'S DISEASE VIRUS

Author
item Reddy, Sanjay
item Lupiani, Blanca
item Silva, Robert
item Lee, Lucy
item Witter, Richard

Submitted to: World Poultry Congress Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 8/20/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Five overlapping cosmid clones were constructed that contain the entire genome of a very virulent strain (Md5) of Marek's Disease Virus (MDV). Transfection of chicken or duck embryonic fibroblasts with the five cosmids resulted in the production of infectious MDV. Generation of recombinant MDV using cosmid DNAs will be useful for studying the function of viral genes in MDV replication and transformation. Furthermore, manipulation of the MDV genome might allow one to produce deletion mutant vaccines or a virus that encodes foreign antigens for use as a polyvalent live virus vaccine.