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United States Department of Agriculture

Agricultural Research Service

Title: Analysis of Chicken Aflp by Cloning and Conversion to Sts Markers

Authors
item Knorr, Christopher - MICHIGAN STATE UNIVERSITY
item Cheng, Hans
item Dodgson, Jerry - MICHIGAN STATE UNIVERSITY

Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 6, 2001
Publication Date: N/A

Interpretive Summary: Genetic maps are the basic tools for identifying genes of agricultural importance. The power of a map is determined by both the utility of the genetic marker and the number of markers. This paper describes how it is possible to increase the utility of a specific type of genetic marker, thus, enhancing the power of a genetic map. This is important to the scientific community as it extends the applicability of the existing genetic maps. Ultimately, genetic maps will enable poultry breeders to generate superior chickens resulting in cheaper and safer poultry products.

Technical Abstract: AFLP fingerprint mapping has been shown to be a useful approach to analyze genetic polymorphism in chickens and other domestic animals. It is often desirable to convert AFLP bands to sequence tagged site markers, in particular, so that AFLP-based linkage information can be integrated with recombinant DNA clone-based maps. Furthermore, this can provide a clearer picture of the source of AFLP sequence polymorphisms in animal genomes. Sixteen chicken (EcoRI/TaqI-based) AFLP bands were excised from gels, reamplified, cloned, and analyzed. All inserts sequenced proved to be EcoRI-TaqI fragments, which confirms that unlabeled TaqI- TaqI AFLP fragments do not amplify well and therefore do not significantly contaminate labeled AFLP bands. For eight of the AFLP, the cloned fragment was used to probe Southern blots of AFLP reactions, confirming that the predominant DNA clone indeed was the polymorphic fragment. Flanking regions of selected AFLP fragments were isolated using Vectorette cloning. The results obtained suggest that the most common source of our chicken AFLP is sequence polymorphism at or near the TaqI site.

Last Modified: 9/21/2014
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