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United States Department of Agriculture

Agricultural Research Service

Title: Experiences with Genetic Typing Techniques to Track Salmonella and Campylobacter Through An Integrated Poultry Operation

Authors
item Bailey, Joseph
item Hiett, Kelli
item Stern, Norman

Submitted to: Australian Food Microbiology Conference
Publication Type: Proceedings
Publication Acceptance Date: December 20, 2000
Publication Date: N/A

Technical Abstract: Identifying Salmonella or Campylobacter on a ready-to-eat or raw processed product only alerts the producer that there is a problem. Effective implementation of a HACCP or pathogen intervention program requires an understanding of the ecology and epidemiology of the pathogens of concern. Requirements for genetic tracking methodologies and strengths and weaknesses of different genetic typing systems including RAPD, RFLP, AFLP, PFGE, and serotyping will be reviewed. Practical experiences with DNA sequencing and ribotyping of isolates from large scale poultry industry epidemiology studies will be highlighted to demonstrate the tracking and ability to determine the genetic relatedness of Salmonella and Campylobacter isolates from the hatchery through grow-out, transportation, and the processing environment.

Last Modified: 7/24/2014
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