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Title: FUSARIC ACID (FA), OTHER ALKYL-PICOLINLIC ACIDS (APA), AND FUMONISIN (FB1) IN FUSARIUM THAPSINUM AND F. MONILIFORME: GC/MS ANALYSIS

Author
item Porter, James
item Bacon, Charles
item Wray, Emma
item KULDAU, G - PENNSYLVANIA STATE UNIV
item LESLIE, J - KANSAS STATE UNIV

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/1/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary: No interpretive summary required for abstract. Presented at the Xth International meeting of the International Union of Pure and Applied Chemists (IUPAC), Symposium on Mycotoxins and Phycotoxins, Sao Paulo, Brazil, May 21-25, 2000.

Technical Abstract: FA (5-butyl-picolinic acid) is produced by most Fusarium species and is a phytotoxin and mycotoxin. Research suggest FA has a unique role in Fusarium-intermediary metabolism, in symptomatic or asymptomatic Fusarium-related activities in plants, and animal toxicity. Previously, we reported FA is synergistic with FB1 in the egg test and contaminated feeds toxic to poultry contained FA, 9,10-dehydro-FA (DHFA), 10-hydroxy-FA (10OHFA), with trace amounts of FB1. Present studies show F. thapsinum and F. moniliforme produce varying amounts of the above, with three other unknown APAs, that frequently occur in Fusarium- contaminated agricultural products. To further understand the APAs' potential in food and feed while Fusaria are in planta, the above were measured in five strains of F. thapsinum (FB1 synthesis is nominal in these strains), in F. moniliforme (FmA), two FA-mutant strains (FmB; FmC), and F. semitectum. GC/MS analyses of the trimethyl-silyl-esters (TMS) of the three unknowns (i.e., APA1, APA2, APA3) suggest they are analogs of FA (i.e., amu fragmentation patterns similar to FATMS, DHFATMS, and 10OHFATMS-2). In all F. thapsinum, FA was in minor conc. (range 27-663 micro-g/g corn), whereas 10OHFA was the major APA (2.5- 31xFA). In FmA: APA1>10OHFA>APA2>FA>DHFA>APA3 (micro-g/g corn). On corn and synthetic medium, FA in FmA>FmB>FmC and only FA was detected in FmC. The abundance of these APAs in culture and field samples warrant further research on their role in Fusarium-plant interactions.