Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: May 25, 2001
Publication Date: May 25, 2001
Citation: Zhu, J.J., Lillehoj, H.S., Allen, P.C., Van Tassell, C.P., Sonstegard, T.S., Chang, H.H., Emara, M.G. 2001. A locus significantly affecting disease resistance to avian coccidiosis identified with genome scan using microsatellite markers. BARC Poster Day.
To map quantitative trait loci (QTL) affecting disease resistance to avian coccidiosis, three hundred and fourteen F2 offspring were produced from a cross between two commercial broiler lines different in resistance to the disease. The F2 offpsring were inoculated with sporulated oocysts of Eimeria maxima. Five disease-associated traits were measured post-infection. Oocyst shedding was the best parameter for evaluating the status of genetic resistance based on statistical analysis of the traits. One hundred and sixteen microsatellite markers, covering 80% of the entire chicken genome with an average marker interval of 25cM, were used for genotyping of F1 parents and F2 offspring. Statistical analysis with SOLAR program based on variance-component and multipoint linkage analysis displayed a locus significantly (LOD=3.07) affecting oocyst shedding. The generic effect of this locus was mostly additive, and the heriability of oocyst shedding at the locus was 0.37. The genomic scan also identified four loci potentially affecting growth. The results will provide the groundwork for further investigation to confirm and to fine map the QTL affecting disease resistance to avian coccidiosis.