|Sangtong, V - ISU|
|Middle, C - ISU|
Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: March 18, 2001
Publication Date: March 18, 2001
Citation: SANGTONG, V., MIDDLE, C., SCOTT, M.P. TRANSGENIC MAIZE LINES EXPRESSING A WHEAT GLU1-DX5 HIGH MOLECULAR-WEIGHT GLUTENIN. MAIZE GENETICS CONFERENCE ABSTRACTS. 2001. http://www.maizegdb.org/cgi-bin/displayrefrecord.cgi?id=315074. Technical Abstract: We have developed lines of transgenic maize containing the Glu1-Dx5 gene from wheat. Lines derived from four independent transformation events exhibit reduced pollen transmission of the transgene. We have also examined the inheritance of the bar transgene that was introduced by co-bombardment with the 1Dx5 transgene, and found that it co-segregates with the Glu1-Dx5 transgene, and exhibits reduced pollen transmission as well. Repeated cycles of selecting Glu1-Dx5 expressing kernels, planting them and self-pollinating the resulting plants has led to the development of lines that produce near-isogenic kernels, 50% of which express the transgene and 50% of which do not. This allows us to determine the effect of the transgene on a trait by comparing kernels from the same ear. In this way, genotypic and environmental effects on comparisons between the expressing and non-expressing kernels are minimized. We have compared the nitrogen and carbon content of pairs of near-isogenic kernels and found that kernels producing the high-molecular weight glutenin contain about 40% more nitrogen.