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United States Department of Agriculture

Agricultural Research Service

Title: Aflp Analysis to Investigate the Parasitic Ability of the Soybean Cyst Nematode, Heterodera Glycines

item Atibalentja, Ndeme - U OF ILL, URBANA
item Noel, Gregory
item Domier, Leslie

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: August 24, 2001
Publication Date: N/A

Technical Abstract: Fifty H. glycines populations were obtained by subjecting populations of races 1 through 6, 9, and 14 to several cycles of selection on each of six sources of resistance to H. glycines, Peking, Cloud, PI88788, PI89772, PI209332, and PI90763. The selected nematode populations were then evaluated, using 15 different AFLP primer combinations, for potential markers associated with parasitic ability. A number of DNA fragments specific to some of the races have been identified. In particular, a 142-bp DNA fragment that is specific to race 5 of H. glycines has been isolated, cloned, and sequenced. Studies are underway to assess the potential of this fragment as a diagnostic probe to identify populations of race 5 from among other field populations of H. glycines.

Last Modified: 4/21/2015
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