Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: October 5, 2001
Publication Date: N/A
Technical Abstract: Single Nucleotide Polymorphisms (SNPs) are the most abundant form of DNA sequence variation, and are useful for genetic mapping, genetic diversity and evolution studies in plants. In a previous study, a mean of 1.69 and 3.94 SNPs per kbp was discovered in coding and non-coding gene sequences, respectively, based on sequence comparisons of 25 genotypes representative of N. American cultivated soybean (Glycine max). In the current study our objective was to further define the pattern of DNA sequence variation within G. max, G. soja (wild soybean), and G. tomentella (a perennial Glycine species). A total of 32 fragments of genes totaling 20 kb were sequenced in each of 52 G. max, 24 G. soja and 20 G. tomentella genotypes from diverse geographic origins. Compared with 98 SNPs and 89 different haplotypes previously found in the 32 fragments in the 25 N. American cultivated soybean germplasm, a total of 164 SNPs and 172 different haplotypes were observed in the 52 G. max or 24 G. soja genotypes. Of the 164 SNPs, 105 SNPs were found in both G. max and G soja, while 16 were unique to G. max and 43 unique to G. soja. Of the 172 different haplotypes, 89 haplotypes were observed in both G. max and G. soja while 23 were unique to G. max and 60 unique to G. soja. Many SNPs were discovered in G. tementella, only a few of which were present in G. max or G. soja, indicating that the pattern of DNA variation in G. tementella is very different from that in G. max and G. soja.