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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Analysis of Hessian Fly Resistance in Durum

Authors
item Williams, Christie
item Jones, Margaret - USDA-ARS (RESIGNED)
item Collier, Chad
item Liang, Chengzhi - PURDUE UNIV. ENTOMOLOGY

Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: January 1, 1997
Publication Date: N/A

Technical Abstract: Roughly half of the 27 known Hessian fly resistance loci in wheat were derived from tetraploid durum. Our lab is pursuing three strategies to study durum-derived resistance loci. 1) Synteny Mapping. The position on wheat chromosome 2B of one of these loci, H20, is being mapped with respect to clones that were previously mapped to 3 different insect resistance loci in rice. Results from this mapping will establish syntenic relationships between the H20 region of wheat and the orthologous region in rice. 2) AFLP Mapping. Resistance loci from five new sources of durum wheat were introgressed onto the hexaploid cultivar 'Cardinal'. Crosses between these five new resistant lines indicated that each contains a single unique resistance gene of durum origin. We are currently combining bulk segregant analysis and genetic mapping of F3 families with AFLP markers to determine the genomic location of the strongest of these genes conferring resistance to biotype L of the Hessian fly. 3) Differential Display. A sequence was recovered, from H9 plants, that was expressed during an incompatible interaction with biotype L Hessian fly. This sequence showed 80% homology to a gene that is turned on by BTH (benzo [1, 2, 3] thiadiazole-7-carbothioic acid S-methyl ester) induction of systemic acquired resistance in wheat.

Last Modified: 10/25/2014
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