Submitted to: Plant Animal and Microbe Genomes Conference
Publication Type: Abstract Only
Publication Acceptance Date: January 10, 2002
Publication Date: January 10, 2002
Citation: MOORE, S.L., PAYTON, P.R., GIOVANNONI, J.J. DEVELOPMENT AND UTILIZATION OF TOMATO MICROARRAYS FOR THE SOLANACEAE. PLANT ANIMAL AND MICROBE GENOMES CONFERENCE. 2002. Technical Abstract: Tomato has long served as the model system for examining climacteric fruit ripening, resulting in the amassing of substantial information and resources regarding the biology of this agronomically important plant. One of the most interesting aspects of the availability of such tools is the possibility of exploiting this knowledge to benefit other species for which hless information (and resources) are currently available. We describe here the construction of a cDNA microarray for analysis of tomato fruit ripening and development. An initial ripening profile is currently being used to establish a baseline of gene expression throughout the ripening process. Additionally, we have demonstrated the potential use of this tomato array as a tool for identifying novel gene functions in other members of the Solanaceae family. Hybridization of leaf and fruit cDNA probes to the tomato chips were used to identify differentially expressed genes in tomato, tobacco, potato, petunia, bell pepper, and eggplant. Selected gene expression profiles were confirmed by RNA gel blot analysis. This information, coupled with corresponding physiological variation among developmental stages or phenotypes will eventually provide specific targets for genetic manipulation in tomato, as well as other members of the family Solanaceae.