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United States Department of Agriculture

Agricultural Research Service

Title: Long Term Survival and Infectivity of Salmonella Choleraesuis

Authors
item Gray, Jeffrey
item Cray, Paula

Submitted to: International Symposium on Epidemiology and Control of Salmonella in Pork
Publication Type: Proceedings
Publication Acceptance Date: September 1, 2001
Publication Date: November 1, 2001
Citation: Gray, J.T., Cray, P.J. 2001. Long term survival and infectivity of salmonella choleraesuis. International Symposium on Epidemiology and Control of Salmonella in Pork. 114. p. 370-374.

Technical Abstract: It is believed that Salmonella Choleraesuis, the host-adapted serotype of swine, does not survive well outside the host. We examined the survival capability as well as the presence of latent DNA of S. Choleraesuis. Feces was collected and pooled on days 2, 4, 7, and 10 post inoculation (PI). Feces was stored in a wet and dry form and survival was measured over 13 months. Salmonella Choleraesuis was recovered from wet feces through 3 months of storage. In a desiccated (dry) form, S. Choleraesuis was recovered for at least 13 months. Direct PCR analysis did not detect S. Choleraesuis subsequent to the final culture recovery for any stored sample. We also examined the infectivity of S. Choleraesuis resident in dry feces. Six or 13 week old pigs were inoculated with dry feces that had been stored either 3 months or 4 months, respectively. Pigs were inoculated either intranasally or by mixing dry feces with the swine ration. Although clinical signs were mild, S. Choleraesuis was widely disseminated among the tissues of all the pigs inoculated. This study demonstrates that S. Choleraesuis remains viable and infective in the environment. Therefore, contaminated fecal matter can serve as a reservoir for S. Choleraesuis as well as other Salmonella spp. Control measures must consider this environmental reservoir as a source of new infections.

Last Modified: 7/28/2014
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