|Park, Young - UNIVERSITY OF WISCONSIN|
Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2002
Publication Date: N/A
Technical Abstract: The watermelon strain of papaya ringspot virus (PRSV-W) and zucchini yellow mosaic virus (ZYMV) are potyviruses that cause significant disease losses in cucumber. Resistances have been identified primarily in exotic germplasm that require transfer to elite cultivated backgrounds. In order to select more efficiently for virus resistances, we identified molecular markers tightly linked to PRSV-W and ZYMV resistances in cucumber. We generated F6 recombinant inbred lines from a cross between Straight 8 and TMG1 (susceptible and resistant, respectively, to both viruses) and studied the segregations of amplified fragment length polymorphisms (AFLPs), randomly amplified polymorphic DNAs, restriction fragment length polymorphisms, and resistances to PRSV-W and ZYMV. A 353-point map of cucumber was generated and resistances to PRSV-W and ZYMV were tightly linked (2.2 cM), mapping to the end of one linkage group. The 5-kb genomic region carrying an AFLP cosegregating with resistance to ZYMV was cloned and sequenced. This region possesses many single nucleotide polymorphisms and small insertion/deletion events; however all polymorphisms were unique to the susceptible parent (Straight 8). We are screening a Melon BAC library to assess the synteny between cucumber and melon across this region and to identify polymorphisms unique to the resistance TMG1 parent.