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United States Department of Agriculture

Agricultural Research Service

Title: Detection of Naturally Occurring Campylobacter in Poultry Rinses by Capacitance Monitoring

Author
item Line, John

Submitted to: International Association for Food Protection
Publication Type: Abstract Only
Publication Acceptance Date: February 22, 2002
Publication Date: July 1, 2002
Citation: Line, J.E. 2002. Detection of naturally occurring campylobacter in poultry rinses by capacitance monitoring. International Association for Food Protection.

Technical Abstract: A conductimetric method utilizing a new selective enrichment broth, Campy- Line Broth + arginine (CLB+ arg), was evaluated for detection and quantification of Campylobacter spp. from poultry carcass rinses. Fresh poultry carcasses (n = 8, post-chill, post-drip) were obtained for each of 6 separate trials and rinsed in buffered peptone water. Module wells containing CLB+arg (2X concentrated) were inoculated with rinse samples fo detection of Campylobacter presence by the Bactometer system; each sample was also directly plated onto Campy-Line agar (CLA) for enumeration of Campylobacter. By monitoring changes in the capacitance signal from the wells, Campylobacter was detected in 100% of the naturally contaminated carcass rinse samples. Samples with higher Campylobacter populations exhibited shorter detection times (DT); thus, a negative correlation was observed between (DT) and number of Campylobacter enumerated by plate count. DTs ranged from about 45 h down to about 15 h for rinses found to contain between about 2 and 1448 Campylobacter cfu/ml, respectively. The correlation coefficient between DT and plate count results was significant; however some variability was observed. Samples removed from module wells and examined by phase contrast microscopy, demonstrated only low numbers of contaminants were present. CLA plates prepared from samples removed from each Bactometer module well (after DTs registered) indicated Campylobacter was the predominant organism causing the significant conductimetric changes. CLB+arg may be useful for rapid detection of Campylobacter in poultry rinse samples; however, the variability in DTs observed would make enumeration by this conductimetric method difficult.

Last Modified: 10/23/2014
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