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United States Department of Agriculture

Agricultural Research Service

Title: Mutation in An Cyaa Homologue in Enterobacter Cloacae Results in Reduced Colonization But Does Not Affect Damping-off on Cucumber

Authors
item ROBERTS, DANIEL
item Lohrke, Scott
item MCKENNA, LAURIE
item BAKER, CON
item Li, W - HUBEI UNIV, CHINA
item Dery, Pierre - USDA, RETIRED
item BUYER, JEFFREY

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: July 30, 2002
Publication Date: N/A

Technical Abstract: Identification of genes involved in seed and root colonization by biocontrol bacteria may allow for strategies for improved ecological fitness, survival, and performance by these strains. <i>Enterobacter cloacae<i/> strain M59, containing a single transposon insertion, was reduced in colonization of cucumber seeds relative to wild-type strain 501R3 but unaffected in control of <i>Pythium ultimum<i/> damping-off on cucumber. DNA sequence analysis indicated that the transposon was inserted in <i>cyaA<i/>, which encodes adenylate cyclase. The growth profile of strain M59 was consistent with a <i>cyaA<i/> mutant. Adenylate cyclase catalyzes the production of cAMP, which in conjunction with CRP, positively regulates several catabolic and other unrelated functions.

Last Modified: 9/10/2014
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