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United States Department of Agriculture

Agricultural Research Service

Title: An Excised-Leaf Inoculation Technique for Evaluating Host-Pathogen Interactions with Dematiaceous Hyphomycetes on Bermudagrass


Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2002
Publication Date: May 1, 2002
Citation: Pratt, R.G. 2002. An excised-leaf inoculation technique for evaluating host-pathogen interactions with dematiaceous hyphomycetes on bermudagrassm [abstract]. Phytopathology 92(Supplement):S66.

Interpretive Summary: None required.

Technical Abstract: An excised-leaf inoculation technique was develped to evaluate interactions of Exserohilum, Bipolaris, Curvularis, and Drechslera spp. with bermudagrass. Sections of leaf blades were excised, placed on water agar, and inoculated with mycelium of pathogens at distal edges. Symptoms were evaluated according to the extent of necrosis that developed down the leaf after 5 days. Symptom severity increased in leaves progressively distal from growing stem tips. Fewer differences in virulence of pathogens were evident in excised leaf tissue than in foliage inoculated with spores. In repeated experiments, significant differences in phenotypic resistance were observed among 40 randomly selected genotypes of bermudagrass when excised leaf tissues were inoculated with E. rostratum. Results suggest that the excised-leaf inoculation technique may be used to identify and compare host resistance to dematiaceous hyphomycetes in individual genotypes of bermudagrass.

Last Modified: 4/22/2015
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