Submitted to: International Coloquim on Invertebrate Pathology and Microbial Control
Publication Type: Proceedings
Publication Acceptance Date: April 12, 2002
Publication Date: August 15, 2002
Citation: HUMBER, R.A. WORKSHOP IN METHODS FOR THE PRESERVATION OF FUNGAL CULTURES. INTERNATIONAL COLOQUIM ON INVERTEBRATE PATHOLOGY AND MICROBIAL CONTROL. 2002. Technical Abstract: There are many possible techniques for the preservation of fungal cultures. This workshop intends to present some of the background information to help guide choices among possible techniques to be used. Individual methods will be illustrated to help make the actual procedures used easier to understand than merely depending on written directions. Variations in possible methods for some of the more popular approaches will be presented since nearly all labs modify the basic methods to suit their own purposes and circumstances; visual documentation of some of these variations should help to make general methods more comprehensible and to provide more choices for methods to preserve fungal cultures. Emphasis will be on (1) various methods to freeze cultures for retention at -20C and in ultra-cold mechanical freezers at -80C or -120C, and in liquid nitrogen dewars; (2) various approaches to freeze drying (lyophilization); and (3) less technically complex methods such as storage in distilled water stasis, under mineral oil, and on silica gel crystals. Discussion by participants about methods used and problems encountered in trying to preserve fungi will be encouraged.