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United States Department of Agriculture

Agricultural Research Service

Title: PROFILING GENE EXPRESSION CHANGES ASSOCIATED WITH EIMERIA INFECTIONS USING HIGH-THROUGHPUT CDNA MICROARRAY

Authors
item Min, Wongi
item Lillehoj, Hyun
item Kim, Sungwon
item Zhu, James
item Beard, Hunter - SOY ALF RES LAB
item Alkharouf, Nadim - SOY ALF RES LAB
item Matthews, Benjamin - SOY ALF RES LAB

Submitted to: Annual Conference on Vaccine Research
Publication Type: Abstract Only
Publication Acceptance Date: June 2, 2002
Publication Date: July 5, 2003
Citation: MIN, W., LILLEHOJ, H.S., KIM, S., ZHU, J., BEARD, H., ALKHAROUF, N., MATTHEWS, B.F. PROFILING GENE EXPRESSION CHANGES ASSOCIATED WITH EIMERIA INFECTIONS USING HIGH-THROUGHPUT CDNA MICROARRAY. Annual Conference on Vaccine Research. 2003.

Technical Abstract: Global transcriptional changes induced in the chicken intestinal intraepithelial lymphocytes (IELs) in response to an intracellular parasitic infection were investigated using nylon and glass cDNA microarrays. Chicken EST cDNAs were selected from the cDNA libraries developed from the activated T-lymphocytes and macrophages (www.chickest.udel.edu). To identify host genes induced by Eimeria infections, we hybridized cDNA microarrays with intestinal mRNA samples obtained from chickens infected with E. acervulina or E. maxima. Among the 2,600 EST clones initially screened, 419 clones were selected on the basis of at least 1.5 folds increased or decreased expression over the uninfected controls. Following infection with E. acervulina or E. maxima, 9.5% and 2.3% of total EST clones tested were increased, and 10.7% and 2.3% were repressed following primary and secondary infections, respectively. In general, E. acervulina infection resulted in a greater change in local gene expression than E. maxima infection. The detailed analysis of local changes in host gene expression following Eimeria infections will enhance our understanding of host-parasite immunobiology leading to protective immunity.

Last Modified: 9/1/2014
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