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United States Department of Agriculture

Agricultural Research Service

Title: Development of 47 New Microsatellite Markers from a Bta6 Library

Authors
item Reed, K - UNIVERSITY OF MINNESOTA
item Ihara, N - JAPAN
item Ponce DE Leon, F - UNIVERSITY OF MINNESOTA
item Sonstegard, Tad
item Smith, Timothy
item Bennett, Gary
item Sugimoto, Y - JAPAN
item Beattie, C - UNIVERSITY OF NEVADA

Submitted to: Animal Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 15, 2002
Publication Date: November 1, 2002
Citation: REED, K.M., IHARA, N., PONCE DE LEON, F.A., SONSTEGARD, T.S., SMITH, T.P., BENNETT, G.L., SUGIMOTO, Y., BEATTIE, C.W. DEVELOPMENT OF 47 NEW MICROSATELLITE MARKERS FROM A BTA6 LIBRARY. ANIMAL BIOTECHNOLOGY JOURNAL. 2002, Vol. 13(2), pp. 195-202.

Interpretive Summary: A prerequisite to fine mapping and marker assited selection (MAS) of quantitative trait loci (QTL) is the development of a genetic map with a high density of informative markers. Although the USDA reference linkage map for bovine chromosome 6 (BTA6) contained more than 50 DNA markers and numerous research groups had used this map to identify QTL, inter-marker resolution has not been sufficient to better resolve these QTL. We used a chromosome specific library to systematically increase DNA marker density for BTA6. Forty-seven new DNA markers were identified. Genotypic data from these 47 markers was integrated into the existing USDA/MARC BTA6 linkage group to nearly double the number of informative markers positioned on the genetic map of BTA6. The nearly two-fold improvement in informative marker density of BTA6 provides a genetic map that will enhance fine mapping of QTL on this chromosome.

Technical Abstract: Chromosome-specific libraries provide a means to isolate genetic markers from specific chromosomal regions. A small-insert BTA6 library, constructed by microdissection, was screened for dinucleotide repeats (CA)15. A total of 47 new microsatellite loci were developed and tested for polymorphism and informativeness using the MARC bovine mapping family.

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